During escape from macrophages, pathogens either secrete pore-forming toxins or induce cell death signalling to rupture host membranes. We still know comparatively little how these interactions are regulated and what mechanisms are involved.

To delineate bacterial egress from host macrophages, we will use single-cell live-cell imaging at a high-temporal resolution. Given that intracellular pathogen escape involves the rupture several membrane, correlative light and electron microscopy will also be utilised to determine the integrity of these membranes.